Chao's Blog

If you are interested in this paper Dynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by interferometric signals temporal analysis, please find it here [1].

In this paper, an new imaging method called dynamic full field optical coherence tomography (D-FFOCT) is proposed, which features in subcellular metabolic contrast in fresh ex vivo tissues. The advantage of higher transverse resolution of FFOCT is taken to obtain intracellular features. In addition, thanks to a higher frame rate and imaging contrast, the proposed method is able to identify different dynamics in tissues.

As mentioned above, D-FFOCT can not only perform long-term acquisitions like conventional OCT by extending the imaging time, but also operate at short timescales (<10 ms) to see subcellular behavior with negligible cellular migration [1]. The time duration is studied in the paper – ‘the time dependence of FFOCT’.

For the optical setup of D-FFOCT, it is the same as that of FFOCT, using a Linnik configuration with broadband light source [2]. However, instead of moving the reference arm in FFOCT, a signal modulation of the path difference is induced by the sample movement in D-FFOCT.

# Reference

[1] C. Apelian, F. Harms, O. Thouvenin, A. C. Boccara, Dynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by interferometric signals temporal analysis. Biomedical Optics Express 7, 1511 (2016).

[2] A. Dubois, L. Vabre, A. C. Boccara, E. Beaurepaire, High-resolution full-field optical coherence tomography with a Linnik microscope. Applied Optics 41, 805-812 (2002).